Yes, DNA polymerase reads the template strand of DNA in a 3′ to 5′ direction. This is crucial for its function in DNA replication.
To understand why this is the case, it’s important to remember that DNA synthesis occurs in the 5′ to 3′ direction. When DNA polymerase synthesizes a new strand of DNA, it adds nucleotides to the 3′ end of the growing strand. Thus, it must read the original template strand from the 3′ end toward the 5′ end. This orientation allows the enzyme to properly align incoming nucleotides with their complementary bases on the template strand.
This reading direction is essential for ensuring accurate DNA replication, as it allows the enzyme to maintain the correct sequence in the newly synthesized strand. Any errors in this process could lead to mutations or other genetic issues.