When two enantiomers are spotted on a TLC (Thin Layer Chromatography) plate, one can generally expect that they will exhibit different Rf (retention factor) values. This is because enantiomers, being non-superimposable mirror images of each other, can interact differently with the stationary phase of the TLC plate as well as with the mobile phase used for elution.
In a typical TLC setup, if both enantiomers are applied to the same plate, they may travel at different rates due to these varied interactions. As a result, one enantiomer might reach a further distance from the origin compared to the other, leading to distinct spots on the TLC plate. Depending on their polarity and the solvent system used, one enantiomer could have a higher Rf value than the other, allowing for their separation and identification.
In conclusion, on a TLC plate, two enantiomers will likely be distinguished by their respective positions, and this can provide valuable information about their relative affinities for the stationary phase during the chromatographic process.